Affinity Labeling Notes: Definitions & Explanations PDF | Download eBooks
Study Affinity Labeling lecture notes PDF with molecular biology definitions and explanation to study What is Affinity Labeling?. Study affinity labeling explanation with molecular biology terms to review molecular biology course for online degree programs.
Affinity Labeling Definition:
Labeling one substance by covalently attaching a reactive compound with specific affinity for the first substance (e.g., labeling the active site of an enzyme by linking a labeled substrate analog covalently to the enzyme)..
Molecular Biology by Robert F. Weaver
Affinity Labeling Notes:
Affinity labeling is a strategy to chemically modify an amino acid residue, within a ligand-binding site in an enzyme. This can happen either at a regulatory allosteric site or at the active site. The process involves a reagent that structurally resembles the natural ligand of the enzyme, but at the same time features a functional group that is capable of covalently reacting with many different amino acid residues.
Keep Learning with Molecular Biology Notes
What is AP Site?
In molecular genetics and biochemistry, an Apurinic or Apyrimidinic site (AP Site), also called an abasic site is a specific ...
What is AP-1?
AP-1 stands for the Activator protein 1, which is a transcription activator that regulates the expression of genes, in response ...
What is Amino Terminus?
Amino terminus or N-terminus is that end of a peptide or a protein primary structure, in which the amino acid ...
What is Amino Tautomer?
An amino tautomer is the normal tautomer of the nucleotides, cytosine or adenine, found in nucleic acids. A tautomer is ...
What is Amber Suppressor?
Amber Suppressor is a mutant allele, coding for a tRNA, bearing an anticodon to recognize the amber codon (UAG), and ...
What are Abortive Transcripts?
Abortive transcripts are short RNA transcripts, about 6 nt long, that are produced at prokaryotic promoters. The release of such ...