Colilert Defined Substrate Test Notes: Definitions & Explanations PDF | Download eBooks
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Colilert Defined Substrate Test Definition:
An assay designed to detect the presence of coliforms in water.
Prescott's Microbiology 9th Edition by Joanne Willey, Linda Sherwood, Christopher J. Woolverton
Colilert Defined Substrate Test Notes:
The colilert test uses Defined Substrate Technology (DST) to detect E.coli and total coliforms simultaneously. MUG and ONPG, which are the two nutrient indicators, are the biggest sources of carbon in colilert and can be broken down by β-galactosidase (coliform enzyme) and β-glucuronidase (E.coli enzyme).
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