Chain Termination DNA Sequencing Method Notes: Definitions & Explanations PDF | Download eBooks

Study Chain Termination DNA Sequencing Method lecture notes PDF with microbiology definitions and explanation to study What is Chain Termination DNA Sequencing Method?. Study chain termination dna sequencing method explanation with microbiology terms to review microbiology course for online degree programs.

Chain Termination DNA Sequencing Method Notes:

Chain Termination method, widely known as the Sanger Sequencing is a method of DNA sequencing, based on the incorporation of selected chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. This method was developed by Frederick Sanger and colleagues in 1977 and first commercialized by Applied Bio systems. It was the most used method used for sequencing for approximately 40 years. Recently this method has been replaced by "Next-Gen" sequencing methods for large scale, automated analyses. Meanwhile, the Chain Termination method is still used for smaller scale projects and for validation of Next-Gen results.

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